EvolutionEcology Essay Example | Topics and Well Written Essays - 500 words

EvolutionEcology - Essay ExamplePaper starts with the speculation demonstrating mitochondrial DNA as primary tool for investigation of evolutionary diversion among closely related species and outgrowth of new approach in terms of PCR-RFLP base rapid and inexpensive techniques to established phylogenitic correlation among opposite species. They also investigated correlation between two different approaches and indicated that data obtained by two different techniques whitethorn not be identical and hence caution must be taken to interpret them. For organisation of hypothesis the authors selected four avian sp. found in North America, those having high rate of hybridization namely, genus Dendroica occidentalis, D. townsendi, D. virens, and D.nigrescens. To investigate above mention hypothesis the first experiment was based on classical RFLP based technique. The total mitochondrial DNA were Isolated and digested with 14 restriction enzymes to obtained band pattern which was subseque ntly analyzed by David L. Swoffords paup* 4.0d64 program for calculation of Nei-Li distances. Similarly, for sequences based analysis, three genes located on two sites on mtDNA were selected and sequenced, namely 681bp cytochrome oxidase I, and 1074bp adenosine triphosphate synthase8 and 6 genes from 30 representative warbler individuals.